Cardiovascular diseases (CVDs) are the leading global cause of mortality. Endothelial dysfunction, an early reversible stage of CVD development, is marked by decreased nitric oxide production linked to Ca2+ influx in endothelial cells.

In this work, we illustrate unconventional approaches towards the fabrication of edge functionalized graphene nanostructures and bidimensional architectures in polymeric and metallic supports, with an outlook towards molecular sensing devices.

Promotor: Jaap Brouwer, Co-promotor: Remus Th. Dame

The goal of the research presented in this thesis was to explore the chemical space of ruthenium(II) photocages, investigate their ability to host potent chemotherapy drugs, and to red shift the activation wavelength of the most promising PACT candidates to conduct biological studies.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Hydrodesulfurization (HDS) is an ubiquitous part of oil refining that ensures that fuels are cleaned of impurities and environment release of pollutants such as SOx and NOx gasses are minimized.

The electrochemical oxygen reduction reaction (ORR) is an essential half-reaction for the utilization of hydrogen as a sustainable fuel, via the conversion of hydrogen to electrons and protons facilitated by the ORR. In the most common fuel cells, the ORR is requires high loadings of non-abundant platinum…

De Universiteit Leiden heeft onderzoeksinstituten gevestigd in Leiden en Den Haag.

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

With increasing carbon dioxide levels in the atmosphere and their detrimental effect on the global climate, modern society needs to push for more renewable energy sources. Storing widely accessible and abundant solar energy in chemical bonds in the form of molecular fuel via artificial photosynthesis…

Promotor: M.T.M. Koper, Co-promotor: J.B.F. Juurlink

Glycosidases (GHs) are enzymes responsible for the degradation of carbohydrates and play many roles in human health and pathophysiology. Often, abnormal levels of glycosidase activity are markedly linked to human pathologies.

Promotor: Prof.dr. J.P. Abrahams, Co-Promotor: N.S. Pannu

Heterogeneous catalysis is one of the fundamental processes of modern life, being common in industrial refinery and hydrogen vehicles, all the way to the living cell. The dissociation of H2 on Cu(111) is an important benchmark system for studying heterogeneous catalysis, with a large and varied amount…

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Producing green energy has become the main goal in our society in the search of reducing or eliminating the carbon emission from fossil fuels.

Promotor: E. Bouwman, Co-Promotor: S. Bonnet

Metals surfaces form a group of effective catalysts for the reaction of small molecules such as hydrogen (H2).

Schistosomiasis is an acute and chronic disease caused by blood dwelling parasitic trematodes of the genus Schistosoma, and it is classified as the second most socioeconomically devastating parasitic disease, second only to malaria.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

Promotor: Prof.dr. J.G.E.M. Fraaije, Co-promotor: A. Kros

Sustainable energy from wind and solar is most readily available near the sea.

A key step in the synthesis of oligosaccharides and glycoconjugates is the formation of the glycosidic bond.

One of the main challenges in the development of an effective anti-cancer vaccine is the generation of an adequate and directed cellular immune response.

Promotor: A. Kros, Co-promotor: R.R.C.L. Olsthoorn

Scheikunde of chemie is de natuurwetenschap die zich bezighoudt met de samenstelling en bouw van stoffen, chemische veranderingen bij bepaalde omstandigheden en de wetmatigheden die je hieruit kunt destilleren.

Monoacylglycerol lipase (MAGL) is the principal enzyme responsible for hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-AG). MAGL inhibition provides several potential therapeutic opportunities, including anti-nociceptive, anti-inflammatory and anti-cancer activity.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

Several single-stranded RNA viruses make use of Xrn1-resistant RNAs in their 3’ untranslated regions of their genome RNAs in order to increase their pathogenicity.

This Thesis focuses on the design and synthesis of ADP-ribosylated compounds that can be applied in biological studies.

The redox-conversion reaction of metal-disulfide and metal-thiolate complexes are important, as they may shed light on electron-transfer reactions that often occur in Nature.

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.

Anionic particles play an important role in biological systems where they fulfil many essential functions for cells.

Metal-organic compounds hold great promise as photoactivated chemotherapy (PACT) agents, leveraging their unique geometric and electronic properties to achieve precise spatiotemporal control over drug activation.

Congres/symposium

The growing demand of energy indicates that global energy resources in the form of fossil fuels will not be sufficient in the future. In order to solve potential future energy problems development of a sustainable hydrogen economy is highly desirable.

This thesis investigates catalytic reactions at the atomic scale using scanning tunneling microscopy (STM), with a focus on Fischer–Tropsch synthesis, a key industrial process that converts hydrogen and carbon monoxide into liquid fuels.

Lipid nanoparticles (LNPs) have enabled the success of mRNA vaccines but remain limited in broader therapeutic use by challenges in delivery efficiency, targeting, and mechanistic understanding.

This thesis investigates innovative and classical strategies in glycoconjugate vaccine development to address the growing threat of multidrug-resistant bacterial infections.

Gaucher disease is a rare metabolic disorder, in which a mutation of the enzyme glucocerebrosidase (GBA1) causes buildup of its substrate glucosylceramide and the deacylated counterpart glucosylsphingosine in lysosomes.

In the emerging field of small-molecule RNA targeting, there is a growing demand for new high-throughput screening methods.

This thesis focuses on developing new chemical synthesis strategies to produce native ADP‑ribosylated biomolecules with precise structural control.

Antibioticaresistentie is een van de grootste bedreigingen wereldwijd voor de volksgezondheid in de komende decennia.

Metal-based prodrugs based on ruthenium(II) polypyridyl complexes have proven to be very suitable for application in both photodynamic therapy (PDT) and photo-activated chemotherapy (PACT).

Promotor: Huub J.M. de Groot, Co-promotor: Francesco Buda