The primary goal of this research is to deepen our understanding of substrate-enzyme interactions in Glycoside hydrolases. For this purpose, Bacillus circulans xylanase (BcX) has been selected as a well-established model to examine the Michaelis complex and glycosyl-enzyme adduct.

Did the Jochids leave their mark on the Grand Duchy, taking into account that the Lithuanian state was one of the main successor states of the Great Horde in the 16thCentury?

Dit programma heeft tot doel de gedigitaliseerde archiefbronnen van de archieven van Kerala en Tamil Nadu toegankelijker te maken voor Indiase en internationale wetenschappers en voor een zo breed mogelijk publiek, in het bijzonder voor de bevolking van Kerala.

Nieuw onderzoek van de National University of Singapore en Leiden laat zien dat chimpansees en bonobo’s net zoveel contrast in hun ogen hebben als mensen, waardoor ze gemakkelijk de richting van iemands blik kunnen volgen.

Onderzoek vindt plaats binnen drie onderzoekscholen: Azië, Midden-Oosten en Religiestudies. Hierbinnen richten we ons op specifieke regio’s en onderwerpen zoals talen en culturen, erfgoed, en de menselijke digitale wereld.

Promotie

Explore how cultural heritage can proactively respond to global crises, from geopolitical conflicts to climate change, through innovative cross-cultural collaboration between Latin American stakeholders and Una Europa.

Do you want your research to fit the rubric of heritage? Is heritage a relevant concept here? These were some of the questions that came up in the lively discussion following twelve five-minute pitches given by Leiden researchers at the ‘Academic Open Mic’ workshop in the Lipsius building on Thursday…

Promotor: H.J.M. de Groot

Decentralized plug and play systems for energy production are the future picture of our society. Artificial photosynthetic systems are used for this purpose.

Promotor: G.A. van der Marel, Co-promotor: D.C. Codée

Carbonaceous asteroids preserve a record of early solar system processes, where minerals and organic molecules, like polycyclic aromatic hydrocarbons (PAHs), offer clues to planetary evolution and prebiotic chemistry.

Vinasse is a major by-product generated by the sugarcane biofuel industry. It is a source of microbes, nutrients and organic matter and often it is recycled as fertilizer.

The research described in this thesis centered on retaining exo-β-glucosidases implicated in health and disease, in particular the human inherited lysosomal storage disorder, Gaucher disease (GD).

Striga hermonthica, commonly known as witchweed, infests major cereal crops in Sub-Saharan Africa causing severe yield losses and threatening the livelihood of millions of resource poor farmers.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

In the current global context, there is a pressing need to address sustainable energy supplies to safeguard our Planet and its ecosystems. The choices made by human society have a significant impact on genetic evolution and climate.

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

This dissertation presents the synthesis and evaluation of antibody recognition for various capsular polysaccharide (CP) fragments of Staphylococcus aureus (S. aureus).

Reducing greenhouse gas emissions is one of the most pressing challenges that humanity faces in the coming decades.

This dissertation explores new chemical strategies for the synthesis of self-adjuvanting peptide conjugates and immunomodulatory ligands aimed at targeted activation of the immune system.

Sorghum (Sorghum bicolor) is a major cereal crop that millions of subsistence farmers in Sub-Saharan Africa (SSA) rely on. However, the scourging impact of the root parasitic weed Striga (Striga hermonthica, a.k.a. “witch-weed”) has remained a major constraint hampering the productivity of sorghum in…

Solar energy is used by photosynthetic organisms to drive energy required cellular processes. Is absorbed by two groups of pigments, located in the LHCs.

Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.

This thesis focuses on developing wafer-scale two-dimensional (2D) materials by combining synthesis, transfer, characterization, and device integration of graphene, hexagonal boron nitride (h-BN), and BN-doped amorphous carbon.

Climate change is one of the largest challenges faced by humanity. To combat this research into alternatives to fossil fuels is ongoing. Dihydrogen is considered a good alternative fuel, since its burning only forms water.

Solar energy harnessed by plants and algae has great potential to be converted into biofuels for future generations.

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

Pseudomonas Aeruginosa is a Gram-negative bacterium which can form biofilms, increasing its resistance against antibiotics and the host immune system. Polysaccharides are an integral part of this biofilm, one of these polysaccharides is called Psl. PslG is a glycosidase, able to cleave this polysaccharide,…

Consequences for sediment stability and seston trapping

Synthesis and application of chemical biology tools to study immunomodulatory signaling lipids.

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

Promotores: Prof.dr. G.A. van der Marel, M. van Heel

Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.

Lysosomal acid glucosylceramidase (GBA1) is a lysosomal enzyme that degrades glucolipids with its main substrate being glucosylceramide (GlcCer). Defects in the GBA1 gene lead to glycosphingolipidosis Gaucher disease (GD), in which the hydrolysis of GlcCer is impaired and therefore, it accumulates in…

The aromatic infrared bands (AIBs) seen throughout the interstellar medium (ISM) are generally thought to be carried by polycyclic aromatic hydrocarbons (PAHs) and their nitrogen analogues (PANHs).

In a typical glycosylation reaction, a donor is activated to form a (variety of) electrophilic species which can react with a nucleophilic acceptor, following a reaction mechanism having both SN1 and SN2 character.

Inspired by natural photosynthesis, perovskite-based photoelectrochemical (PEC) systems are being developed for artificial photosynthesis, aiming to enhance solar-to-hydrogen conversion for green energy.

This thesis describes the use of a combined approach of computational and experimental techniques to gain novel insights to understand the glycosylation reaction and its reactive intermediates.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

Astronomical observations of cold regions in the universe show a rich inventory of ices. Part of these ices may end up on planets like our own, but in that journey they will be exposed to considerable amounts of radiation.

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

This thesis is concerned with the constitution of chiral responsive matrix assemblies that may undergo light-driven conformational changes and self-select vibrations to develop vibronic states for driving semi-classical coherent transfer with nearly 100% yield in a lossless NCAP process.

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

Electron microscopy has become an extremely important techniquein a wide variety of elds.